The LiQuta™ RiboGreen RNA Assay Kit is one of the most sensitive detection kits for quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. LiQuta™ mRNA Reagent enables quantitation of as little as 1 ng/mL RNA (200 pg RNA in a 200 µL assay volume) with a fluorescence microplate reader. The linear range of the LiQuta™ mRNA Reagent extends over three orders of magnitude in RNA concentration (1 ng/ml to 1 µg/ml) using two dye concentrations. The high-range assay allows quantitation of 20 ng/ml to 1 µg/ml RNA, and the low-range assay allows quantitation of 1 ng/ml to 50 ng/ml RNA. The kit can be used to measure encapsulation efficiency and RNA concentration for mRNA-LNP. The assay is well tolerated to common contaminants such as proteins, salts, nucleotides, urea, ethanol, chloroform, detergents, and agarose.
Application
RNA quantitation in solution
Specifications
1. Sample Type: RNA
2. Platform: Fluorescence plate reader or Qubit
3. Linear Range: 1-200 ng RNA
Components
1. LiQuta™ mRNA Reagent: 1 ml, Solution in DMSO
2. LiQuta™ mRNA Buffer: 200 ml
3. RNA Standard: 1 ml, 100 ng/µl in TE buffer
Storage
Store at 2~8°C and protect from light.
Case Study
Figure Left: The high-range assay, the LiQuta™ RiboGreen RNA Reagent was diluted 200-fold.
Figure Right: The low-range assay, the LiQuta™ RiboGreen RNA Reagent was diluted 2000-fold.