LiFluor™ 488 TUNEL Apoptosis Imaging Kit utilizes a dUTP modified with an alkyne, a small bio-orthogonal functional group that enables the nucleotide to be more readily incorporated by TdT than other modified nucleotides such as biotin-dUTP and fluorescein-dUTP. Detection is based on a click reaction, a copper(I)-catalyzed reaction between an azide and an alkyne. The small size of the LiFluor™ azide (MW ∼1,000) compared to that of an antibody (MW ∼150,000) enables effortless penetration of complex samples with only mild fixation and permeabilization required. As a result, compared to assays using other modified nucleotides, the TUNEL Imaging Assay is fast (complete within 2 hours) and detects a higher percentage of apoptotic cells under identical conditions.
Key Features
• Broad application: Suitable for FFPE tissue, frozen tissue, and cells.
• High sensitivity: Detects a higher percentage of apoptotic cells.
• Bright apoptotic signal: Utilizes LiFluor™ dyes, providing a stable, non-photobleaching fluorescent signal.
Specifications
1. Platform: Fluorescence Microscopy
2. Detection Method: Fluorescent
3. Ex/Em: 495/520 nm
Applications
Cell apoptosis analysis
Components
1. TdT reaction buffer: 8 ml
2. TdT enzyme: 100 µl
3. EdUTP: 50 µl
4. Reaction buffer: 6 ml
5. Reaction buffer additive: 50 mg
6. Hoechst 33342: 100 µl
7. DNase I: 20 µl
8. DNase I buffer: 1.5 ml
Storage
Store -20°C and protect from light.
Case Study
Detection of apoptotic cells in mouse tissue using LiFluor™ 488 TUNEL Apoptosis Imaging Kit.