tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
54131
UniProt ID:
P70671
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables DNA-binding transcription factor activity, RNA polymerase II-specific; identical protein binding activity; and promoter-specific chromatin binding activity. Involved in positive regulation of macromolecule biosynthetic process; response to virus; and type I interferon-mediated signaling pathway. Acts upstream of or within several processes, including lipopolysaccharide-mediated signaling pathway; regulation of defense response; and response to exogenous dsRNA. Located in nucleus. Is expressed in several structures, including central nervous system; early conceptus; eye; and genitourinary system. Human ortholog(s) of this gene implicated in brain disease. Orthologous to human IRF3 (interferon regulatory factor 3).
