tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
18104
UniProt ID:
Q64669
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and mitogen-activated protein kinase p38 binding activity. Involved in heart development and positive regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including circulatory system development; leukocyte differentiation; and negative regulation of cell population proliferation. Located in cytoplasm and nucleus. Part of transcription regulator complex. Is expressed in several structures, including eye; genitourinary system; heart; hemolymphoid system; and limb mesenchyme. Human ortholog(s) of this gene implicated in congenital heart disease and ventricular septal defect. Orthologous to human NFATC1 (nuclear factor of activated T cells 1).
