serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
19697
UniProt ID:
Q548Y4
Component:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; actinin binding activity; and ankyrin repeat binding activity. Involved in several processes, including negative regulation of protein sumoylation; positive regulation of canonical NF-kappaB signal transduction; and regulation of DNA-templated transcription. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of extrinsic apoptotic signaling pathway; and positive regulation of interleukin-12 production. Located in chromatin; cytosol; and nucleus. Part of NF-kappaB p50/p65 complex and transcription regulator complex. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; musculature; and skin. Human ortholog(s) of this gene implicated in ductal carcinoma in situ; lung non-small cell carcinoma; lymphoma; and renal cell carcinoma. Orthologous to human RELA (RELA proto-oncogene, NF-kB subunit).
