tissue homogenates, cell lysates or other biological fluids.
Experimental method:
Sandwich
Shelf life:
12 months
Gene ID:
25027
UniProt ID:
P53987
Components:
1. Pre-coated, ready to use 96-well strip plate 1
2. Plate sealer for 96 wells 2
3. Standard 2
4. Diluents buffer: 1×45 mL
5. Detection Reagent A: 1×120 μL
6. Detection Reagent B: 1×120 μL
7. TMB Substrate: 1×9 mL
8. Stop Solution: 1×6 mL
9. Wash Buffer (30× concentrate): 1×20 mL
Background
Enables enzyme binding activity. Involved in negative regulation of ERK1 and ERK2 cascade; negative regulation of reactive oxygen species metabolic process; and positive regulation of insulin secretion. Predicted to be located in mitochondrial inner membrane and mitochondrial matrix. Predicted to be part of protein-containing complex. Predicted to be active in mitochondrion and nucleus. Used to study metabolic dysfunction-associated steatotic liver disease. Biomarker of congestive heart failure; myocardial infarction; obesity; and prediabetes syndrome. Orthologous to human SIRT3 (sirtuin 3).
