LiPure™ DNA Clean-up Kit is designed for the rapid purification of single- or double-stranded DNA from PCR and other enzymatic reactions. The system follows a "bind-wash-elute" procedure and effectively removes primers, nucleotides, enzymes, salts, and other impurities from DNA samples. Its convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, allowing multiple samples to be processed in parallel. The purified DNA is suitable for T-A ligation, sequencing, restriction enzyme digestion, and various other labeling reactions.
Key Features
• Rapid: Purify PCR products in less than 10 minutes.
• Safe: No phenol/chloroform extraction required.
• High-quality: Purified DNA is ready for a wide range of downstream applications.
Specifications
1. Sample Source: PCR products, enzymatic reactions
2. Sample Input: 10-50 μl
3. Elution Volume: 30-50 μl
4. Technology: Silica spin column
Applications
DNA isolation from PCR and other enzymatic reactions.
Components
1. Buffer PB: 10 ml
2. Buffer PW: 15 ml
3. Elution buffer: 15 ml
4. Mini Column: 50×
5. Collection Tube: 50× 2ml
Storage
Store at room temperature
Case Study
Different size DNA fragments were cleaned up with LiPure™ DNA Clean-up Kit to remove primers, nucleotides, and other impurities. DNA was analyzed on a 1% agarose gel.