LiDirect™ DNA Methylation Kit (50 preps)

LiDirect™ DNA Methylation Kit integrates DNA denaturation and bisulfite conversion into a single step. In addition, the kit has been streamlined to achieve high-yield DNA recovery following bisulfite conversion. The Kit features innovative in-column desulfonation technology, eliminating cumbersome DNA precipitation steps while delivering consistent results every time. The kit is optimized to minimize template degradation and DNA loss during treatment and cleanup, ensuring complete conversion of unmethylated cytosines. The recovered DNA is ideal for PCR amplification and downstream applications, including endonuclease digestion, sequencing, microarrays, and more.
 

Key Features

• Complete bisulfite conversion of DNA in less than 2 hours.

• Desulfonation and recovery of bisulfite-treated DNA using a spin column.

• Recovered DNA is suitable for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
 

Specifications

1. Conversion: >99%
2. Elution Volume: ≥20 μl
3. Input: 500 pg – 2 μg of DNA
4. Process Time: 2 hours
5. Recovery: >75%
 

Applications

Library preparation for next generation sequencing, PCR amplification, etc.
 

Components

1. CT Conversion Reagent: 5 Tubes
2. Dilution Buffer: 1.5 ml
3. Dissolving Buffer: 500 µl
4. Binding Buffer: 30 mL
5. Wash Buffer: 6 ml
6. Desulphonation Buffer: 10 ml
7. Elution Buffer: 1.2 ml
8. Mini Column: 50×
9. Collection Tube: 50× 2ml

Storage

Store at room temperature