DNAzol Reagent (100 ml)

DNAzol Reagent is a complete, ready-to-use solution for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. It is a novel guanidine‑detergent lysing solution that permits selective precipitation of DNA from a cell lysate. DNAzol Reagent provides a simple, reliable, and efficient method for DNA isolation from a wide variety of samples. The protocol is fast and allows for the isolation of genomic DNA from a large number of samples, whether of small or large volume.

During isolation, a biological sample is lysed (or homogenized) in DNAzol Reagent, and the genomic DNA is precipitated from the lysate with ethanol. After an ethanol wash, the DNA is solubilized in water or 8 mM NaOH. The entire procedure can be completed in 10–30 minutes, with DNA recovery rates of 70–100%. The isolated DNA can be used without additional purification in applications such as Southern analysis, dot blot hybridization, molecular cloning, and polymerase chain reaction (PCR).
 

Key Features

• Versatile — compatible with a broad spectrum of sample materials.

• Efficient — rapid isolation and high recovery of genomic DNA.
 

Component

1. DNAzol Reagent: 100 ml
 

Storage

Store at room temperature and Protect from light.