Double-stranded RNA (dsRNA) ELISA Kit (96T)

The use of in vitro transcribed RNA for disease prevention or treatment requires large quantities of functional mRNAs with low immunogenicity. Such mRNAs are synthesized primarily using bacteriophage T7 RNA polymerase (T7 RNAP), which efficiently transcribes RNA from DNA templates containing specific promoters. However, previous studies have identified certain by-products generated during in vitro synthesis that can trigger cellular immune responses, including double-stranded RNA (dsRNA)—a proven major trigger of immune signaling pathways.

This kit employs a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the dsRNA content in in vitro transcription systems or synthesized mRNA stock solutions.
 

Key Features

• Critical Application: Enables quality control of in vitro transcribed mRNA used for disease prevention or treatment.

• Key Impurity Detection: Specifically detects dsRNA, a major immunogenic by-product of T7 RNAP-mediated transcription.

• Sample Versatility: Compatible with both in vitro transcription reaction systems and purified mRNA stock solutions.

• Quantitative Result: Provides precise measurement of dsRNA contamination levels.
 

Applications

mRNA quality analysis and impurity detection.
 

Components

1. Pre-coated microplate (coated with anti-dsRNA monoclonal antibody): 12x 8, 96 wells
2. Sample diluent: 2x 30 ml
3. dsRNA (modified) standard (300 ng/ml): 100 µl
4. Concentrated wash buffer (20×): 30 ml
5. Detection antibody (100×): 120 ml
6. Enzyme-labeled reagent diluent: 12 ml
7. Enzyme-labeled reagent (100×): 120 µl
8. TMB substrate solution: 12 ml
9. Sealing film: 4 pcs
 

Storage

Store at 2-8°C and protect from light.