LiPure™ MagBead Plasmid Mini Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with magnetic beads technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Key Features
• Rapid: Purify plasmid DNA in under 30 minutes.
• Safe: No phenol/chloroform extraction required.
• High-quality: DNA is suitable for various downstream applications.
Specifications
1. Sample Source: LB culture
2. Sample Input: 1-6 mL high-copy plasimd, 10 mL low-copy plasimd
3. Elution Volume: 30-100 μl
4. Technology: Magnetic Beads
Applications
Plasimd DNA isolation from 1-6 mL bacterial cultures.
Components
1. Buffer P1: 15 ml
2. Buffer P2: 15 ml
3. Buffer N3: 20 ml
4. Buffer PW: 15 ml
5. Elution buffer: 15 ml
6. RNase A: 150 µl, 10 mg/ml)
7. Magnetic beads: 2.5 ml
Storage
Store at -20°C for RNase A; 2-8°C for magnetic beads; room temperature for other.
Case Study
5 μl of purified pUC18 plasmid was analyzed on a 1% Agarose gel. Plasmid was isolated from 5 ml DH5a cultures harboring the plasmid using LiPure™ MagBead Plasmid Mini Kit.
pUC18 plasmid DNA yield and quality using LiPure™ MagBead Plasmid Mini Kit.