LiScript™ One-Step RT-PCR Kit provides all the necessary reagents for both reverse transcription and PCR amplification to be carried out in a single reaction tube. The RT-PCR Enzyme Mix contains LiScript™ Reverse Transcriptase, Hot-Start Taq DNA Polymerase, and an RNase Inhibitor, enabling highly sensitive and specific RT-PCR from any RNA template. Our proprietary RT-PCR Reaction Mix includes stabilizers and enhancers that optimize both reactions in a "single-step" format. Together with this specially formulated Reaction Mix, the One-Step RT-PCR Kit offers end users an efficient and reliable alternative to conventional two-step sequential RT-PCR.
Key Features
• Single-tube convenience: Combines reverse transcription and PCR amplification in one reaction, reducing handling steps and contamination risk.
• High sensitivity and specificity: LiScript™ Reverse Transcriptase and Hot-Start Taq DNA Polymerase ensure robust performance across a wide range of RNA templates.
• Optimized proprietary buffer: The 5× RT-PCR Reaction Mix contains stabilizers and enhancers specifically formulated to balance both enzymatic reactions in a single step.
• Time-efficient workflow: Eliminates the need for separate cDNA synthesis and setup of a second PCR reaction, streamlining your RT-PCR process.
• Reliable results: Delivers consistent performance as a practical alternative to traditional two-step protocols.
