LiPure™ Plasmid Mini Kit enables the isolation of up to 30 µg of high-quality plasmid DNA from 1–6 mL of bacterial culture in less than 30 minutes. The purification process employs the alkaline-lysis method and is streamlined using Spin Column technology into three quick steps: Bind, Wash, and Elute. The purified plasmid DNA is immediately ready for a wide range of downstream applications, including routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing.
Key Features
• Rapid: Purify plasmid DNA in under 30 minutes.
• Safe: No phenol/chloroform extraction required.
• High-quality: DNA is suitable for various downstream applications.
Specifications
1. Sample Source: LB culture
2. Sample Input: 1-6 mL high-copy plasmid, 10 mL low-copy plasmid
3. Elution Volume: 30-100 μL
4. Technology: Silica spin column
Applications
Plasmid DNA isolation from 1-6 mL bacterial cultures.
Components
1. Buffer P1: 15 ml
2. Buffer P2: 15 ml
3. Buffer N3: 20 ml
4. Buffer PW: 15 ml
5. Elution buffer: 15 ml
6. RNase A: 150 µl, 10 mg/ml)
7. Mini Column: 50×
8. Collection Tube: 50× 2 ml
Storage
RNase A at -20°C and other at RT
Case Study
5 μl of purified pUC18 and pUC19 plasmids were analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5a cultures harboring the plasmid using LiPure™ Plasmid Mini Kit.
DNA yield and quality using LiPure™ Plasmid Mini Kit.