LiQuant™ microRNA Assay Kit is ideal for quantification of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay kit is highly selective for small RNA over rRNA or large mRNA. The assay kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The assay kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted microRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer. The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
Key Features
• Higher sensitivity: 100 times more sensitive than NanoDrop.
• Broad dynamic range: over four orders of magnitude.
• More accuracy: unaffected by the presence of proteins, free nucleotides.
• Easy to use: mix-and-read format.
• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.
Applications
Quantification of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA
Specifications
1. Sample Type: microRNA
2. Platform: Fluorescence plate reader or fluorometer
3. Linear Range: 0.5-150 ng microRNA
Components
1. LiQuant™ microRNA Reagent: 1 ml
2. LiQuant™ microRNA Buffer: 250 mL
3. microRNA Standard #1: 1 ml
4. microRNA Standard #2: 1 ml
Storage
Store at -20°C and protect from light.
Other Size
| Product Name | Cat. # | Price | View |
| LiQuant™ microRNA Assay Kit (200 assays) | M0134-02 | $69 |  |
Case Study
Figure Left: Quantitation of microRNA with LiQuant™ microRNA Assay Kit using fluorescence plate reader.
Figure Right: Quantitation of microRNA with LiQuant™ microRNA Assay Kit using Qubit® Fluorometer.