LiQuant™ Protein Assay Kit is designed for easy and accurate quantitation of protein samples. The kit is highly selective for proteins and remains accurate in the presence of reducing reagents, though large amounts of detergent are not well tolerated and can be used as a replacement for Qubit protein assay kit.
Key Features
• High selectivity for proteins: Accurate quantitation even in the presence of reducing reagents.
• Broad contaminant tolerance: Well tolerates reducing reagents (e.g., DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, and DNA (detergents not tolerated).
• Complete all-in-one kit: Includes concentrated assay reagent, dilution buffer, and pre-diluted protein standards.
• Flexible sample volume: Accepts 1–20 µL of sample.
• Wide dynamic range: Accurate for initial sample concentrations from 12.5 µg/mL to 5 mg/mL.
• Low protein-to-protein variation: Consistent results across different protein types.
• Convenient workflow: Performed at room temperature; signal stable for 3 hours.
• Versatile format: Adaptable for use in microplates, tubes, or cuvettes.
Specifications
1. Platform: Fluorescence plate reader or Qubit fluorometer
2. Assay Range: 12.5 µg/ml to 5 mg/ml
Applications
Protein quantitation
Components
1. LiQuant™ Protein Reagent: 300 µl
2. LiQuant™ Protein Buffer: 50 ml
3. Protein Standard #1: 1 ml
4. Protein Standard #2: 1 ml
5. Protein Standard #3: 1 ml
Storage
Store at 2-8°C and protect from light.
