RNAzol RT Reagent (100 ml)

RNAzol RT Reagent is a ready-to-use solution designed for isolating high-quality total RNA and small RNA from samples of human, animal, plant, bacterial, and viral origin. It is a monophasic solution containing phenol, guanidine isothiocyanate, and other proprietary components that facilitate the isolation of RNA species of both large and small molecular sizes. During sample homogenization, RNAzol RT Reagent effectively inhibits RNase activity while disrupting cells and dissolving cellular components, thereby preserving RNA integrity. The reagent also supports the simultaneous processing of a large number of samples.
 

Key Features

• No chloroform‑induced phase separation is required to obtain pure RNA. Simply add water to remove DNA, proteins, polysaccharides, and other contaminants.

• The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.

• RNAzol RT can isolate total RNA, or separate large and small RNA fractions. The large RNA fraction contains rRNA and mRNA, while the small RNA fraction contains tRNA, small RNA, and microRNA.

• The isolated RNA is ready for RT‑PCR, qRT‑PCR, microarrays, poly A+ selection, northern blotting, RNase protection assays, and other molecular biology applications.
 

Component

1. 2× LiTaq™ Fast PCR Master Mix (+dye): 20×1 ml
 

Storage

Store at 2-8°C and protect from light.