The mitochondrion plays a vital role in both apoptotic and necrotic cell death. The mitochondrial permeability transition pore (mPTP) is a nonspecific channel formed by components from the inner and outer mitochondrial membranes and is involved in the release of mitochondrial components during cell death.
The Mitochondrial Permeability Transition Pore (mPTP) Flow Cytometry Assay Kit is based on established experimental methods for detecting mitochondrial transition pore opening. This assay employs calcein AM—a colorless, nonfluorescent esterase substrate—and CoCl₂, a quencher of calcein fluorescence, to selectively label mitochondria.
Key Features
• Direct mPTP Measurement: Provides a direct method for assessing mitochondrial permeability transition pore opening suitable for flow cytometry.
• Flow Cytometry Compatible: Designed for high-throughput, quantitative analysis of mPTP opening at the single-cell level.
• Quantitative & Multiparametric: Enables simultaneous measurement of mPTP opening with other flow cytometry markers.
