Unlock high-quality DNA from challenging formalin-fixed, paraffin-embedded (FFPE) tissue sections. LiPure™ FFPE DNA Extraction Kit pairs a standard xylene/ethanol deparaffinization method with an advanced lysis buffer and Proteinase K digest, effectively reversing formalin crosslinking to recover both short and long DNA fragments. Utilizing a convenient spin-column workflow, the kit quickly washes away cellular debris and proteins without toxic organic extractions or tedious alcohol precipitation. Get pure, amplifiable DNA ready for next generation sequencing (NGS), PCR, and other critical downstream applications.
Key Features
• Fast Protocol: Rapid spin-column purification takes less than 20 minutes post-lysis.
• Safe Workflow: No toxic organic extractions or time-consuming ethanol precipitation.
• Sequencing-Ready: High-purity DNA optimized for PCR and Next Generation Sequencing.
Specifications
1. Sample Source: FFPE tissue
2. Sample Input: Up to 25 mg
3. Elution Volume: 50-100 μl
4. Technology: Silica spin column
Applications
Genomic DNA isolation from formalin-fixed, paraffin-embedded (FFPE) tissue sections.
Components
1. Buffer ATL: 15 ml
2. Buffer DL: 15 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE : 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Mini Column: 50×
8. Collection Tube: 50× 2ml
Storage
Store at -20°C for proteinase K; Room temperature for other.
Case Study
Genomic DNA from FFPE samples was isolated using LiPure™ FFPE DNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.
Genomic DNA yield and quality using LiPure™ FFPE DNA Extraction Kit.