Nucleic Acid Assay Kits
Nucleic Acid quantitation of purified DNA/RNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequencing, PCR, Cloning and DNA/RNA transfection.
LiQuant™ Nucleic Acid Assay Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our LiQuant™ assay kits are ideal for quantitating DNA or RNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.
Selection Guide
| Product | Sensitivity | Application |
| dsDNA HS Assay Kit | 0.2-100 ng | dsDNA quantitation of PCR products, viral DNA and samples for subcloning |
| 1X dsDNA HS Assay Kit | 0.2-100 ng | dsDNA quantitation of DNA samples for Next Generation Sequencing |
| dsDNA BR Assay Kit | 2-1000 ng | dsDNA quantitation of genomic and miniprep DNA samples |
| 1X dsDNA BR Assay Kit | 2-1000 ng | dsDNA quantitation of genomic and miniprep DNA samples |
| ssDNA Assay Kit | 1-200 ng | ssDNA quantitation of oligo, primers, denatured DNA, PCR products |
| RNA HS Assay Kit | 5-100 ng | RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures |
| RNA BR Assay Kit | 20-1000 ng | RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures |
| microRNA Assay Kit | 0.5-150 ng | microRNA and siRNA quantitation |
| RiboGreen RNA Assay Kit | 1-200 ng | Useful for determining mRNA encapsulation efficiency |

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Case Study

Figure Left. Selectivity for dsDNA using LiQuant™ dsDNA HS Assay Kit in the presence of an equal mass of RNA. The DNA quantitation kit show superior accuracy for detecting dsDNA compared with absorbance-based quantitation method, especially at relatively low DNA concentrations.
Figure Right. Comparison of accuracy of DNA quantitation between the LiQuant™ dsDNA HS Assay Kit and absorbance-based quantitation method.
