LiPure™ Magbead Blood DNA Extraction Kit enables rapid total DNA isolation from 10–250 µL of fresh or frozen anticoagulated whole blood. This kit can also be used for genomic DNA preparation from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and other body fluids. It allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extraction and time-consuming steps such as isopropanol or ethanol precipitation have been eliminated. The isolated DNA is ready for applications including PCR, Southern blotting, and restriction enzyme digestion.
Key Features
• Fast: DNA purification in less than 30 minutes.
• Safe: No phenol/chloroform extraction required.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Fresh and frozen anticoagulated whole blood, buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and other body fluids.
2. Sample Input: 10-250 μl
3. Elution Volume: 50-100 μl
4. Technology: Magnetic Beads
Applications
Total DNA isolation from fresh and frozen anticoagulated whole blood.
Components
1. Buffer AL: 15 ml
2. Buffer DW1: 15 ml
3. Buffer DW2: 15 ml
4. Buffer AE: 15 ml
5. Magnetic Beads: 2.5 ml
Storage
Store at 2-8°C for magnetic beads; Room temperature for other.
Case Study
Genomic DNA was isolated from 250 µl fresh anticoagulated whole blood using LiPure™ Magbead Blood DNA Extraction Kit from blood. DNA was analyzed on a 1% agarose gel.