LiPure™ MagBead Cell DNA Extraction Kit enables rapid total DNA isolation from cultured cells. The kit can be processed either manually or on an automated platform. Phenol/chloroform extraction and time-consuming steps such as isopropanol or ethanol precipitation have been eliminated. The isolated DNA is ready for applications including PCR, Southern blotting, and restriction enzyme digestion.
Key Features
• Fast: DNA purification in less than 30 minutes.
• Safe: No organic extraction or ethanol precipitation.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Cultured cells
2. Sample Input: 5× 10^6 cells
3. Elution Volume: 50-100 μl
4. Technology: Magnetic Beads
Applications
Total DNA isolation from cultured cells.
Components
1. Buffer AL : 15 ml
2. Buffer DW1 : 15 ml
3. Buffer DW2: 15 ml
4. Buffer AE: 15 ml
5. Magnetic Beads: 2.5 ml
Storage
Store at 2-8°C for magnetic beads; Room temperature for other.
Case Study
Genomic DNA from different cell lines was isolated using LiPure™ MagBead Cell DNA Extraction Kit. Eluted DNA was analyzed on a 0.8% agarose gel.