Unlock high-quality DNA from challenging formalin-fixed, paraffin-embedded (FFPE) tissue sections with ease. LiPure™ MagBead FFPE DNA Extraction Kit combines a standard deparaffinization method with an advanced lysis buffer and Proteinase K digest, effectively reversing crosslinking to recover both short and long DNA fragments. Utilizing a streamlined magnetic bead workflow, this kit rapidly binds and purifies DNA while clearing cellular debris without toxic organic extractions or tedious alcohol precipitation. Experience minimal hands-on time and get pure, reliable DNA ready for demanding applications like SNP analysis, sequencing, and genotyping.
Key Features
• Fast Protocol: Rapid spin-column purification takes less than 20 minutes post-lysis.
• Safe Workflow: No toxic organic extractions or time-consuming ethanol precipitation.
• Sequencing-Ready: High-purity DNA optimized for PCR and Next Generation Sequencing.
Specifications
1. Sample Source: FFPE tissue
2. Sample Input: Up to 25 mg
3. Elution Volume: 50-100 μl
4. Technology: Magnetic Beads
Applications
Genomic DNA isolation from formalin-fixed, paraffin-embedded (FFPE) tissue sections.
Components
1. Buffer ATL: 15 ml
2. Buffer DL: 15 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE : 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Magnetic Beads: 2.5 ml
Storage
Store at -20°C for proteinase K; 2-8°C for magnetic beads; Room temperature for other.
Case Study
Genomic DNA from FFPE samples was isolated using LiPure™ MagBead FFPE DNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.
Genomic DNA yield and quality using LiPure™ MagBead FFPE DNA Extraction Kit.