LiPure™ MagBead Tissue DNA Extraction Kit enables rapid and reliable isolation of high-quality genomic DNA from fresh or frozen animal tissue samples. The kit can be used either manually or on an automated platform. The system combines the reversible nucleic acid-binding properties of paramagnetic particles with the proven efficiency of LIFESCT's buffer chemistries, providing a fast and convenient method for DNA isolation from a variety of samples. Phenol/chloroform extraction and time-consuming steps such as isopropanol or ethanol precipitation have been eliminated. The use of paramagnetic particles yields high-quality DNA suitable for direct use in most downstream applications, including next generation sequencing, qPCR, PCR, and microarrays.
Key Features
• Fast: DNA purification in less than 20 minutes post-lysis.
• Safe: No phenol/chloroform extraction required.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Fresh and frozen tissues, paraffin-embedded tissues, cells, mouse tail snips, blood, and buccal swabs.
2. Sample Input: 10-25 mg tissues, 5×10^6 cultured cells
3. Elution Volume: 50-100 μl
4. Technology: Magnetic Beads
Applications
Total DNA isolation from fresh and frozen animal tissues.
Components
1. Buffer ATL: 15 ml
2. Buffer DL: 15 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE: 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Magnetic Beads: 2.5 ml
Storage
Store at -20°C for proteinase K; 2-8°C for magnetic beads; Room temperature for other.
Case Study
Plasma samples were purified using LiPure™ MagBead Tissue DNA Extraction Kit.
Genomic DNA yield and quality using LiPure™ MagBead Tissue DNA Extraction Kit.