LiPure™ Plant DNA Extraction Kit is designed for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant and fungal tissues. Up to 100 mg of wet samples or 20 mg of dry samples can be processed in less than 60 minutes. The kit utilizes a proprietary buffer system to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant samples. No organic extraction or time-consuming precipitation steps are required. Purified DNA is suitable for PCR, restriction enzyme digestion, and hybridization techniques.
Key Features
• Fast: DNA purification in less than 60 minutes.
• Safe: No organic extraction or ethanol precipitation.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Plant and fungal tissues
2. Sample Input: 100 mg wet tissues, 20 mg dry tissues
3. Elution Volume: 50-100 μl
4. Technology: Silica spin column
Applications
Total DNA isolation from plant and fungal tissues.
Components
1. Buffer AP1: 25 ml
2. Buffer AP2: 10 ml
3. Buffer AP3: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE: 15 ml
6. Mini Column: 50×
7. Collection Tub: 50× 2ml
Storage
Store at room temperature
Case Study
Genomic DNA from various plant sources was isolated using LiPure™ Plant DNA Extraction Kit. Eluted DNA was analyzed on a 0.8% agarose gel.