LiPure™ Stool DNA Extraction Kit provides fast, reliable extraction of total DNA from fresh or frozen stool samples. Process up to 200 mg of sample in less than 30 minutes with minimal hands-on time.
Our specially formulated lysis buffer enables direct cell lysis while efficiently removing PCR inhibitors and DNA-degrading substances via simple centrifugation. The cleared supernatant is then purified using an advanced DNA spin column, eliminating the need for hazardous phenol-chloroform extraction or tedious alcohol precipitation. The resulting high-quality DNA is ready for immediate use in demanding downstream applications.
Key Features
• Rapid Results: Complete workflow in under 30 minutes.
• Safe & Clean: Eliminates organic extractions and ethanol precipitation.
• High Yield & Quality: Fully compatible with PCR, sequencing, genotyping, Southern blotting, and restriction digestion.
Specifications
1. Sample Source: Stool
2. Sample Input: 200 mg stool sample
3. Elution Volume: 50-100 μl
4. Technology: Silica spin column
Applications
Total DNA isolation from fresh or frozen stool samples.
Components
1. Buffer ASL: 50 ml
2. Buffer DL: 25 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE : 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Mini Column: 50×
8. Collection Tube: 50× 2ml
Storage
Store at -20°C for proteinase K; Room temperature for other.
Case Study
Genomic DNA from three human stool samples was isolated using LiPure™ Stool DNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.