LiPure™ Universal DNA Extraction Kit provides rapid, reliable extraction of high-quality genomic DNA from a diverse range of sample types—including fresh or frozen animal tissues, cultured cells, whole blood, serum, plasma, saliva, swabs, sperm, and dried blood spots.
Utilizing advanced silica spin-column technology, the streamlined "lyse-bind-wash-elute" workflow takes less than 20 minutes post-lysis. This convenient format eliminates time-consuming alcohol precipitation and hazardous organic extractions (such as phenol/chloroform) while enabling efficient parallel processing of multiple samples. The resulting ultra-pure DNA is ready for immediate use in demanding downstream applications, including PCR, sequencing, genotyping, Southern blotting, and restriction enzyme digestion.
Key Features
• Fast: Complete purification in under 20 minutes post-lysis.
• Safe: No organic extractions or ethanol precipitation required.
• High-Quality: Yields intact, high-purity DNA optimized for diverse downstream assays.
Specifications
1. Sample Source: Animal tissues and cells, blood, serum, plasma, saliva, swab, sperm, blood spot.
2. Sample Input: 100 mg
3. Elution Volume: 50-100 μl
4. Technology: Silica spin column
Applications
Genomic DNA isolation from a variety of sample sources including fresh or frozen animal tissues and cells, blood, serum, plasma, saliva, swab, sperm, blood spot.
Components
1. Buffer ATL: 15 ml
2. Buffer DL: 15 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE : 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Mini Column: 50×
8. Collection Tube: 50× 2ml
Storage
Store at -20°C for proteinase K; Room temperature for other.
Case Study
Genomic DNA from different samples was isolated using LiPure™ Universal DNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.