Prestained Protein Marker consists of high-purity and pre-stained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. This ready-to-load protein ladder is formulated in Laemmli buffer with 0.1% SDS, requiring no denaturation or reduction steps prior to SDS-PAGE or western blot applications.
The bands display in Tris-Glycine gel range from 15-154 kDa (~12, ~22, ~28, ~38, ~50, ~62, ~70, ~78, ~113, ~154 kDa), with orange-red band at 70 kDa, blue band at 12 kDa, and the others can bind almost all types of antibodies (except chicken antibodies). The marker can bind the antibody to the target protein at the same time and develop color by ECL or other methods.
Key Features
• Ready-to-use: supplied in a loading buffer for direct loading on gels; no need to boil.
• Sharp bands: color-coded bands of similar intensity for easy visualization.
• Quality tested: each lot evaluated by SDS-PAGE and Western blotting.
• Membrane-compatible: colored bands transfer to membranes for Western blotting.
Applications
1. Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
2. Monitoring protein transfer onto membranes after western blotting.
3. Sizing of proteins on SDS-polyacrylamide gels and western blots.
Component
1. Western Protein Marker, 12-154 kDa: 2× 250μl
Storage
Store at -20°C
Other Size
Images

