Prestained Protein Marker consists of high-purity and pre-stained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. This ready-to-load protein ladder is formulated in Laemmli buffer with 0.1% SDS, requiring no denaturation or reduction steps prior to SDS-PAGE or western blot applications.
The bands display in Tris-Glycine gel range from 30-209 kDa (~30, 38, 50, 62, ~70, 78, ~105, ~113, 154, 209 kDa), of which 70 kDa is the red pre-stained band, 30 kDa and 105 kDa are the blue pre-stained bands to monitor of electrophoresis and transfer efficiency. The seven recombinant proteins (38, 50, 62, 78, 113, 154, 209 kDa) can bind almost all types of antibodies (except chicken antibodies) and can bind antibodies simultaneously with the target protein and can be colourised by ECL or other means.
Key Features
• Ready-to-use: supplied in a loading buffer for direct loading on gels; no need to boil.
• Sharp bands: color-coded bands of similar intensity for easy visualization.
• Quality tested: each lot evaluated by SDS-PAGE and Western blotting.
• Membrane-compatible: colored bands transfer to membranes for Western blotting.
Applications
1. Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
2. Monitoring protein transfer onto membranes after western blotting.
3. Sizing of proteins on SDS-polyacrylamide gels and western blots.
Component
1. Western Protein Marker, 30-209 kDa: 2× 250μl
Storage
Store at -20°C
Other Size
Images

