Gene knockout (KO) cell lines constructed using the CRISPR system provide invaluable tools for functional genomics, protein characterization, and target validation. A single cell carrying the desired mutation can be expanded into a cloned KO cell line, creating a homogeneous and reproducible experimental system. These cloned cell lines are crucial for understanding the functional consequences of gene deletion, studying signaling pathways, and validating the specificity of biological reagents. LifeSct's custom KI cell line service provides clients with experimental design, optimized delivery methods, clone screening, and rigorous validation.
 

Key Features

• Exclusive innovative technique, Gene-editing efficiency improved by 10-20 times.
• Fast and precise identification, saving 4 weeks to screen positive clones.
 

Cell Line Type

Various types of cells including tumor cell lines, regular cell
 

Service Type

RNP Method, Prime Editor, Base Editing, Antibiotics-based Knock
 

Turnaround

6-8 weeks
 

Deliverables

Single-cell clone