Stable cell lines are indispensable for functional genomics and the development of animal disease models, as they circumvent the inherent variability and transient expression characteristic of standard transfection. LifeSct offers a sophisticated platform for the construction of both overexpression and knockdown stable cell lines. By utilizing high-titer lentiviral transduction combined with stringent antibiotic selection, we achieve high integration efficiency and consistent transgene expression across a broad spectrum of mammalian cell types.
 

Key Features

• LifeSct offers a one-stop solution covering primer synthesis, gene synthesis, viral packaging, and cell engineering, enabling fast and efficient project delivery.
• Our stable overexpression cell lines are generated using lentiviral transduction combined with antibiotic selection, enabling the establishment of stable cell lines across a wide range of cell types.
 

Service Type

Overexpression cell line (qPCR and Western blot Validation)
 

Turnaround

80-90 business days
 

Deliverables

Target, Control and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell.