Custom Stable Cell Lines
Stable cell lines are essential for functional genomics and animal disease modeling because they eliminate the variability and transient expression of standard transfection. LIFESCT provides a robust platform for engineering both overexpression and knockdown stable cell lines. Using high-titer lentiviral transduction and stringent antibiotic selection, we deliver high integration efficiency and consistent transgene expression across diverse mammalian cell types.
Key Features
• LIFESCT offers a one-stop solution covering primer synthesis, gene synthesis, viral packaging, and cell engineering, enabling fast and efficient project delivery.
• Our stable overexpression cell lines are generated using lentiviral transduction combined with antibiotic selection, enabling the establishment of stable cell lines across a wide range of cell types.
Service Options
| Service Type | Description | Deliverables | Lead Time |
| Overexpression Cell Lines | • Gene construction & lentivirus packaging (gene≤3kbp) • qPCR and western blot validation |
Target, control ,and wild-type cells, >10^6 cells/tube *2 tubes for each type of cell | 70-80 business days |
| Knockdown RNAi Cell Lines | • Gene construction & lentivirus packaging • qPCR and western blot validation |
Target, control ,and wild-type cells, >10^6 cells/tube *3 tubes for each type of cell | 70-80 business days |
Case Study
Fluorescence imaging and Western blot analysis demonstrating stable overexpression of the target gene in KYSE-150 cells.