Baculovirus-Insect Cell Protein Expression
Baculovirus-insect cell expression system is widely used to express various recombinant proteins, due to its advantages of high expression level of foreign genes, easy expression of heteromultimeric proteins, and safety. Recombinant proteins expressed by insect cells usually have correct post-translational modifications and biological activities. Insect cells can be cultured in suspension, easy to scale up and culture, which is conducive to large-scale expression of recombinant proteins. The insect cell/baculovirus expression system is very suitable for expression of recombinant proteins located in the cell and nucleus. For toxic proteins and glycoproteins, they can be secreted into the medium by adding secretion signal peptides, such as HBM. LifeSct has established the insect cell expression platform to provide high-quality and affordable eukaryotic protein expression services.
Specifications
| Process | Description | Delivery Form | Turnaround Time |
| 1. Gene synthesis and subcloning (optional) | Gene Synthesis with codon optimizationSubcloning | Construct report | 5-8 business days |
| 2. Virus generation | Recombinant Bacmid DNA generation and transfection of insect cell P1 stock (low titer) and P2 stock (high titer) generation, the titer of virus will be determined by quantitative-PCR Protein expression verification |
Virus generation report, virus stock is available upon request | 5-8 business days |
| 3. Expression evaluation | Infection of insect cells SDS-PAGE and/or Western blot |
Expression report | 5-8 business days |
| 4. 1 L expression & purification | 1 L insect cell expression One-step purification* QC by SDS-PAGE and Western blot |
Purified proteins QC report | 8-12 business days |
