Adenovirus Packaging
Recombinant adenovirus (Ad) is a replication-defective adenovirus vector system. Adenovirus packaging relies on cell lines expressing E1 genes, such as HEK-293 and HEK-293A, as the adenoviral packaging is defective in the early transcription of replication genes E1 and E3 (DE1, DE3), with the E3 gene being non-essential for viral production. LIFESCT's adenovirus packaging system (Ad5 type) offers distinct advantages compared to the admax system. Notably, it facilitates easier detection of false positives of linearized plasmid recombination in the pronucleus. Additionally, the linearized adenovirus plasmid can be utilized in cell lines expressing E1, such as HEK-293 and HEK-293A. Our adenovirus vectors meet the rigorous requirements for both cell infection and in vivo experiments, ensuring reliable results for your research endeavors.
Key Features
• High infection efficiency and expression levels enable adenovirus vectors to infect nearly all types of cells.
• High titer ensures that purified adenovirus is well-suited for in vivo experiments.
• Ease of amplification significantly reduces costs.
• Non-integration into the host cell genome ensures high safety.
Selection Guide
| Scale | Package | Turnaround time | Price |
|
Medium |
Customized Over-expression Adenovirus (Gene ≤ 3kb), Deliverables: ≥ 2x1010 PFU/ml, 1 ml |
55 business days | Quote |
| RNAi/sgRNA Adenovirus-single target(sgRNA only , no Cas9 gene), Deliverables: ≥ 2x1010 PFU/ml, 1 ml |
45 business days | Quote | |
| RNAi/sgRNA Adenovirus-three targets(sgRNA only, no Cas9 gene), Deliverables: ≥ 2x1010 PFU/ml, 1 ml |
45 business days | Quote | |
| sgRNA-Cas9 Adenovirus(sgRNA+Cas9), Deliverables: ≥ 2x1010 PFU/ml, 1 ml |
55 business days | Quote | |
| miRNA-up/down-Adenovirus, Deliverables: ≥ 2x1010 PFU/ml, 1 ml |
45 business days | Quote | |
|
Large |
Customized Over-expression Adenovirus (Gene ≤ 3kb), Deliverables: ≥ 1011 PFU/ml, 1 ml |
60 business days | Quote |
| RNAi/sgRNA Adenovirus-single target(sgRNA only , no Cas9 gene), Deliverables: ≥ 1011 PFU/ml, 1 ml |
50 business days | Quote | |
| sgRNA-Cas9 Adenovirus(sgRNA+Cas9), Deliverables: ≥ 1011 PFU/ml, 1 ml |
60 business days | Quote |
Deliverables
| Custom Adenovirus, delivered to specification: Medium Scale: ≥ 2x 1010PFU/ml, 5× 200μl Large Scale: ≥ 109 TU/ml, 5× 200μl |
| Free: Control virus Medium Scale: ≥ 2x 1010PFU/ml, 5× 200μl Large Scale: ≥ 109 TU/ml, 5× 200μl |
| Polybrene, 10 mg/ml, 1× 200 μl (Free) |
| Dry ice/-80 ° C, avoid repeated freezing and thawing |