Custom TurboMice
TurboMice™ is next-generation mouse model generation technology, which harnesses optimized tetraploid complementation to deliver fully homozygous mouse models in just 2–4 months—60% faster than traditional breeding methods. Unlike conventional approaches that require 6–12 months of tedious breeding cycles, our process starts with precision editing of embryonic stem cells (ESCs) for targeted gene knockouts, floxing, or multi-locus modifications. We then use tetraploid complementation to develop these ESCs directly into experiment-ready mice, skipping the need for chimeric F1 and F2 generations. This method ensures 100% genotype certainty from the F0 generation, eliminating the guesswork of heterozygous screening and saving researchers thousands of hours spent on colony management.
Key Features
• The world's first platform to industrialize Tetraploid Complementation Technology, enabling one-step generation of homozygous models (directly addresses the chimerism pain point associated with traditional methods).
• Outstanding performance in complex models, enable one-step acquisition of CKO/CKI Homozygotes (e.g., mutti-gene editing, humanzied genomic replacement).
• Customized Genetic Models with Guaranteed Homozygosity—No Breeding Required.
• Delivery cycle: only 2-4 months (over 60% faster than traditional methods).
Service Options
| Service Type | Features | Lead Time |
| Custom KO Mice | • Homozygous mice • No chimeras, no breeding selection required |
2~4 months |
| Custom KI Mice | • Any target site you wish to knock in • Homozygous mice • No chimeras, no breeding selection required |
3~5 months |
| Custom CKO Mice | • Homozygous mice • No chimeras, no breeding selection required |
3~5 months |
| Custom Humanized Mice | • Humanizing point mutations • Humanizing the entire gene • Humanizing multi-gene loci • Homozygous mice • No chimeras, no breeding selection required |
3-5 months |
| Custom Point Mutant Mice | • Homozygous mice • No chimeras, no breeding selection required |
3~5 months |
| Custom Transgenic Mice | • Homozygous mice • No chimeras, no breeding selection required |
3~5 months |
| Custom Muti-site Gene Editing Mice | • Homozygous mice • No chimeras, no breeding selection required |
4~6 months |
Case Study
Traditional transgenic K18-ACE2 mice are generated through random exogenous insertion, which precludes precise editing and results in a model lacking tissue specificity. In contrast, TurboMice™ humanized ACE2 models allow for organ-specific expression (Figures C and D), more accurately mimicking clinical disease manifestations.
By optimizing enhancers, we successfully upgraded our humanized ACE2 mouse model four times in just one year. Humanized ACE2 expression has increased steadily with each iteration and now nears endogenous levels—a feat that would take traditional methods significantly longer and cost much more to achieve.
Publications
1. Highly cooperative chimeric super-SOX induces naive pluripotency across species.
Publication: Cell stem cell
2. Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models.
Publication: Cell Research
3. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target.
Publication: Cell Research