Custom Gene Editing Cell Lines
EZ-editor™ technology has been developed for efficient and precise gene editing in cell lines. It integrates a unique gRNA design algorithm tailored to genomic features, optimized editing parameters for thousands of cell lines, accurate assessment of cell pool editing efficiency, enhanced single-clone formation techniques, and high-throughput genotyping of small cell populations. Compared to traditional methods, EZ-editor™ boosts gene-editing efficiency by 10–20×, delivering faster and more reliable results for your research. LIFESCT's gene editing cell lines service provides clients with experimental design, optimized delivery methods, clone screening, and rigorous validation.
Key Features
• Exclusive innovative technique, gene-editing efficiency improved by 10-20 times, with knockout efficiency up to 97%.
• Rapid turnaround: monoclonal knockin cell lines delivered in as fast as 10 weeks.
• Fast and precise identification, saving 4 weeks to screen positive clones.
Service Options
| Service Type | Deliverables | Lead Time |
| Custom KO Cell Lines | 1. ≥1 homozygous single clone (>10^6 cells/vial, 2 vials per clone) 2. Wild-Type Cells (>10^6 cells/vial, 2 vials) 3. Project reports |
4~5 weeks |
| Custom KI Cell Lines | 1. ≥1 homozygous single clone (>10^6 cells/vial, 2 vials per clone) 2. Wild-Type Cells (>10^6 cells/vial, 2 vials) 3. Project reports |
10~12 weeks |
| Custom Point Mutant Cell Lines | 1. ≥1 homozygous single clone (>10^6 cells/vial, 2 vials per clone) 2. Wild-Type Cells (>10^6 cells/vial, 2 vials) 3. Project reports |
6-8 weeks |
Case Study
Custom NINJ1 knockout (NINJ1-/-) THP-1 cells. Single-cell–derived clones were validated by PCR, Sanger sequencing, and Western blot, confirming frameshift mutations at the genomic DNA level.
