Affinity Maturation
Affinity maturation refers to the process of enhancing antibody affinity via in vitro experiments when the initial affinity is insufficient for downstream development. Conventional methods include constructing saturation mutagenesis libraries followed by high-throughput screening based on mammalian cell expression. We guarantee a more than fivefold improvement in the affinity of the delivered antibody.
Key Features
• AI-assisted antibody back-mutation design significantly improves efficiency and saves time.
• Unbiased CDR saturation mutagenesis combined with precise affinity data.
• Guaranteed affinity improvement—enhanced by several folds, reaching nanomolar levels or higher.
Specifications
| Item | Description | EDT |
| 1. Sequence analysis and design | • Unbiased CDR saturation mutagenesis based on client requirements | 1 week |
| 2. Single-point saturation mutation library display | • Single-point saturation mutation library construction • High-throughput mammalian cell secretory expression |
3~4 weeks |
| 3. Affinity testing | • Culture supernatant ELISA/BLI affinity ranking • Key mutation site identification |
|
| 4. Combination mutation screening | • Combination mutation design • Plasmid construction, transient expression and purification • BLI affinity ranking • Biacore affinity validation |
3~4 weeks |
Deliverables
1. 2–3 affinity-matured antibody sequences(at least one with >5-fold affinity improvement)
2. Cloned plasmids
3. Affinity maturation report
4. 2–3 antibody products (1 mg each)
5. COA report