Recombinant Rabbit mAb
Rabbit antibodies typically recognize a broader range of epitopes and exhibit higher affinity than mouse antibodies. As a strong complement to mouse monoclonal antibodies, they are widely used in research, diagnostics, and therapeutics. Utilizing phage display technology, LIFESCT has developed a cutting-edge transient expression platform designed for monoclonal antibodies (mAbs), bispecific antibodies, and VHH antibodies. We deliver gram-level expression, improved stability, and reduced aggregation, accelerating your biopharmaceutical development from discovery to preclinical research.
Key Features
• Rapid screening for high-quality monoclonal antibodies
• High efficiency and high affinity
• Professional execution with strict quality control
• Ultra-competitive pricing powered by our in-house gene synthesis and sequencing platforms
Specifications
1. Immunization
| Item | Description | EDT |
| Antigen Testing | Antigen quality tested by SDS-PAGE or other methods | |
| Animal Immunization | Two New Zealand White rabbits, 3–5 rounds of immunization | 6-8 weeks |
2. Fab/ScFv Library Construction
| Item | Description | EDT |
| cDNA Synthesis | Collection of immune cells, total RNA extraction, and reverse transcription to synthesize cDNA | 1 week |
| Plasmid Construction | Antibody gene fragments amplified by PCR using cDNA as a template and cloned into a vector | 4 weeks |
| Transformation | Constructed vectors electroporated into E. coli strains | |
| Library Capacity Evaluation | 1. After recovery, transformed strains are serially diluted,plated, and positive colonies counted 2. 10 positive monoclonal colonies are selected for plasmid extraction and restriction enzyme validation 3. Based on colony counts and validation results, library capacity is calculated. Ensure ≥ 5×10⁸ clones/library |
2 weeks |
| Antibody Library Production | Phage display antibody library obtained via helper phage rescue | 1 week |
3. Antibody Screening
| Item | Description | EDT |
| Antigen Testing | Antigen quality tested by SDS-PAGE or other methods | |
| Antibody Screening | 4 rounds of panning, with antibody affinity > 10-7 | 3~4 weeks |
| Phage Binding Assay | 1. Phage amplification 2. ELISA Testing 3. Phage DNA extraction 4. Phage DNA sequencing |
2 weeks |
| Antibody Detection | 5–10 phage clones with distinct sequences selected for soluble antibody expression and ELISA validation | 2 weeks |
| Deliverables | Project reports (including sequencing results), and cDNA of antibody gene fragments |
4. Additional Services
| Item | Description | EDT |
| Additional Immunization in Animals | Increase the number of immunized animals | |
| Antibody Expression | Full-length antibody transient expression in Expi293 or CHO cells | 2-4 Weeks |