LiPure™ MagBead Bacteria DNA Extraction Kit provides a rapid and easy method for isolating high-quality genomic DNA from a wide variety of gram-positive and gram-negative bacterial species. The kit can be processed manually or on an automated platform. The buffer system is optimized to enable direct cell lysis followed by selective binding of DNA to magnetic beads. Three rapid wash steps remove trace salts and protein contaminants, after which DNA is eluted in water or a low ionic strength buffer. Purification requires no phenol or chloroform extraction, no alcohol precipitation, and involves minimal handling. DNA purified with this kit is ready for most downstream applications, including PCR, sequencing, genotyping, Southern blot analysis, and restriction enzyme digestion.
Key Features
• Fast: DNA purification in less than 20 minutes post-lysis.
• Safe: No organic extraction or ethanol precipitation.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Bacterial cells
2. Sample Input: 1× 10^9 cells
3. Elution Volume: 50-100 μl
4. Technology: Magnetic Beads
Applications
Genomic DNA isolation from gram-positive and negative bacterial cells.
Components
1. Buffer ATL: 15 ml
2. Buffer DL: 15 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE : 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Lysozyme: 100 mg
8. Magnetic Beads: 2.5 ml
Storage
Store at -20°C for proteinase K & lysozyme; 2-8°C for magnetic beads; Room temperature for other.
Case Study
Genomic DNA from various bacteria was isolated using LiPureTM Magbead Bacteria DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel.