LiPure™ MagBead Plant DNA Extraction Kit is designed for the rapid and reliable isolation of high-quality genomic DNA from plant, fungal, and other tissues that are particularly difficult to lyse or have very high polysaccharide content. This kit is suitable for both manual and fully automated high-throughput preparation of genomic, chloroplast, and mitochondrial DNA. The buffer system is specifically formulated to minimize co-purification of polysaccharides and polyphenols. No organic extraction or time-consuming precipitation steps are required. Purified DNA is suitable for PCR, restriction digestion, next-generation sequencing, and hybridization applications.
Key Features
• Fast: DNA purification in less than 20 minutes post-lysis.
• Safe: No organic extraction or ethanol precipitation.
• High-quality: Purified DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Source: Plant and fungal tissues
2. Sample Input: 100 mg wet tissues, 20 mg dry tissues
3. Elution Volume: 100-200 μl
4. Technology: Magnetic Beads
Applications
Total DNA isolation from plant and fungal tissues.
Components
1. Buffer AP1: 25 ml
2. Buffer AP2 : 10 ml
3. Buffer AP3: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE: 15 ml
6. Magnetic Beads: 2.5 ml
Storage
Store at 2-8°C for magnetic beads; Room temperature for other.
Case Study
Genomic DNA from various plant sources was isolated using LiPure™ Magbead Plant DNA Extraction Kit. Eluted DNA was analyzed on a 0.8% agarose gel.