LiPure™ MagBead Stool DNA Extraction Kit extracts high-quality total DNA from fresh or frozen stool samples quickly and easily. This high-efficiency kit processes up to 200 mg of stool per sample with minimal hands-on time. Our advanced lysis buffer drives direct cell lysis while rapidly clearing PCR inhibitors and DNA-degrading substances through simple centrifugation. The DNA is then purified using a high-performance magnetic bead workflow—no toxic phenol-chloroform extractions, no alcohol precipitation, and no hassle. Get pure, reliable DNA ready for downstream applications like PCR, sequencing, genotyping, and restriction digestion.
Key Features
• Fast Workflow: Magnetic bead protocol takes less than 30 minutes.
• Safe & Non-Toxic: No hazardous organic extractions or ethanol precipitation.
• Downstream Ready: High-purity DNA suitable for sequencing, PCR, and blotting.
Specifications
1. Sample Source: Stool
2. Sample Input: 200 mg stool sample
3. Elution Volume: 50-100 μl
4. Technology: Magnetic Beads
Applications
Total DNA isolation from fresh or frozen stool samples.
Components
1. Buffer ASL: 50 ml
2. Buffer DL: 25 ml
3. Buffer AW1: 15 ml
4. Buffer AW2: 15 ml
5. Buffer AE : 15 ml
6. Proteinase K: 1 ml, 20 mg/ml
7. Magnetic Beads: 2.5 ml
Storage
Store at -20°C for proteinase K; 2-8°C for magnetic beads; Room temperature for other.
Case Study
Genomic DNA from three human stool samples was isolated using LiPure™ MagBead Stool DNA Extraction Kit. Eluted DNA was analyzed on a 1% agarose gel.