RNAzol BD Reagent (100 ml)

RNAzol BD Reagent provides an unsurpassed yield of total RNA, large RNA (>150–200 bases), or small RNA (<150–200 bases). Small RNA constitutes approximately 15–30% of the total RNA. RNAzol BD offers the opportunity to isolate and investigate all RNA present in whole blood, unlike other methods that capture only a small fraction.

RNAzol BD Reagent is a monophasic solution containing acidic phenol and guanidine thiocyanate. A blood, plasma, or serum sample is lysed in RNAzol BD, and RNA is separated from DNA, proteins, polysaccharides, and other cellular components by phase separation. Pure RNA is precipitated from the aqueous supernatant by adding isopropanol. The RNA pellet is then washed and solubilized. To process 0.5 mL of blood, use 1 mL of RNAzol BD Reagent; the entire procedure takes less than one hour.
 

Key Features

• The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.

RNAzol BD isolates total RNA, or separates large RNA and small RNA into distinct fractions. The large RNA fraction contains rRNA and mRNA, while the small RNA fraction contains tRNA, small RNA, and microRNA.

The isolated RNA is ready for RT‑PCR, qRT‑PCR, microarrays, poly A⁺ selection, northern blotting, RNase protection assays, and other molecular biology applications.
 

Applications

• RNA sequencing

• Gene expression analysis

• RT‑PCR & qRT‑PCR

• Microarrays

• poly A+ selection
 

Components

1. RNAzol BD Reagent: 100 ml
2. BCP: 8 ml
 

Storage

Store at room temperature