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  • Early Stage Apoptosis Assay Kits

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Early Stage Apoptosis Assay Kits

Early Stage Apoptosis Assay Kits

Caspase Activity Assays

Caspase-3/7 Apoptosis Assay Kit provides a simple and reliable method for detecting apoptosis by measuring caspase-3/7 activity. The kit can be used to continuously monitor caspase-3/7 activity in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader.

• Simple and reliable: Directly measures caspase-3/7 activity for apoptosis detection.
• Continuous monitoring: Tracks activity in cell extracts and purified enzyme preparations.
• Non-fluorescent substrate: Z-DEVD-R110 yields bright fluorescence upon enzymatic cleavage.
• Specificity control: Ac-DEVD-CHO inhibitor confirms signals are caspase-3/7-dependent.
• Quantitative capability: R110 reference standard allows precise product quantification.
 

Product Name Cat. # Price View
Caspase-3/7 Apoptosis Assay Kit (100 assays) C0046 $219
Caspase-3 Substrate (5 mg) C0151 $439
Caspase-3 Inhibitor (5 mg) C0152 $279


Mitochondrial Membrane Potential Assays

LiFluor™ JC-1 Assay Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry.

• Simple: add, incubate, and read format, no wash needed.
• Flexibility: suitable for flow cytometry, fluorescence microscope, and plate reader.
• Fast: results in as little as 30 minutes.
 

Product Name Cat. # Price View
LiFluor™ JC-1 Assay Kit (100 assays) C0047 $199
JC-1 Dye (5 mg) C0063 $199
JC-9 Dye (5 mg) C0163 $199
Red Mitochondrial Superoxide Indicator (50 μg) C0162 $69
Rhodamine 123 (25 mg) C0062 $129
Orange CMTMRos Mitochondria Stain (1 ml) C0059 $129
Red CMXRos Mitochondria Stain (1 ml) C0060 $129
Green FM Mitochondria Stain (1 ml) C0159 $129
Red FM Mitochondria Stain (1 ml) C0160 $129
Deep Red FM Mitochondria Stain (1 ml) C0161 $129


Case Study

Caspase Activity Assay

Detection of apoptosis activity in Jurkat cells using the Caspase-3/7 Apoptosis Assay Kit.

Mitochondrial Membrane Potential Assay

Detection of cell apoptosis with LiFluor™ JC-1 Assay Kit. Jurkat cells treated with 10 μM camptothecin for 4 hr (right panel) or untreated (left panel). Cells were stained with JC-1 reagent, then analyzed by flow cytometry.

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