LiPure™ Gel Extraction Kit utilizes proprietary spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels, with high recovery rates (>80%). The kit employs a specialized binding buffer system that dissolves the gel slice and facilitates DNA binding to the spin column. Following the binding step, three rapid wash steps are performed, and the DNA is eluted using the provided elution buffer. The purified DNA is immediately ready for various downstream applications, including ligations, PCR amplification, restriction enzyme digestion, cloning, and labeling reactions.
Key Features
• Rapid: Recover DNA from an agarose gel in just 15 minutes.
• Safe: No phenol/chloroform extraction required.
• High-quality: DNA is suitable for a wide range of downstream applications.
Specifications
1. Sample Source: Agarose gel slice
2. Sample Input: Up to 20 μg DNA
3. Elution Volume: 30-50 μl
4. Technology: Silica spin column
Applications
DNA isolation from agarose gel.
Components
1. Buffer GDB: 25 ml
2. Buffer PW: 15 ml
3. Elution buffer: 15 ml
4. Mini Column: 50×
5. Collection Tub: 50× 2ml
Storage
Store at room temperature
Case Study
Comparison of LiPure™ vs Brand Q, Brand T and Brand A for 5.4 bp gel extraction (Right).
Purification of PCR products for 50 bp、486 bp、1 kb、2 kb、4.5 kb、7.5 kb、10 kb、12 kb (Left).