The Lentiviral Packaging Kit streamlines the packaging of third-generation lentivectors into high-titer viral particles. This advanced system enables robust expression of your target construct across a broad spectrum of mammalian cells. Engineered to the highest biosafety standards, the system separates the lentiviral Rev gene onto a distinct vector. This design eliminates the risk of reverse recombination and prevents the generation of replication-competent lentiviruses (RCL), ensuring your applications are both potent and secure.
Key Features
• Optimized Platform: Leverages a high-efficiency, HIV-based packaging system.
• High Titers: Yields consistently elevated viral concentrations with ease.
• Broad Compatibility: Delivers most third-generation lentivector expression constructs.
Components
1. Packaging Plasmid Mix: 50 μl (0.5 μg/μl)
2. Control Plasmid: 50 μl (0.25 μg/μl)
3. LiFect2K™ Transfection Reagent: 250 µl
Storage
Packaging plasmid mix and control plasmid: at -20°C; Transfection reagent: at 4°C